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. Author manuscript; available in PMC: 2020 Oct 21.
Published in final edited form as: Curr Biol. 2019 Oct 10;29(20):3478–3487.e4. doi: 10.1016/j.cub.2019.08.063

Figure 4. Expression of clock genes in wild-type and Opn5−/− outer ear.

Figure 4.

Pinnae were harvested from mice that were housed in either a 12h:12h LD cycle (A-F) or in constant darkness for at least 36 hours (G-L). Transcript levels were determined using ΔΔCt RT-PCR. White and black boxes above charts indicate times of light or darkness in an LD cycle or the subjective LD cycle for groups in DD. Wild-type data are shown using blue symbols and data from Opn5−/− using red symbols. Charts (M, N) show data for liver from wild-type and Opn5−/−, LD and DD as labeled. Each chart point indicates mean±SEM for n=4 in each group. To assess significant differences across a time-course, we used the ANOVA with Tukey post-hoc statistical test: p < 0.05 is indicated by the hash symbol (#). The same statistical test was used to assess difference between time-courses: p < 0.05 between groups is indicated by an asterisk (*). N = 4 mice for each point.