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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell. 2019 Mar 7;177(2):352–360.e13. doi: 10.1016/j.cell.2019.01.042

Figure 5.

Figure 5.

Proteomics and ICP-OES data showing the enrichment of ion transporters and increased levels of cellular magnesium content in the L22* mutant strain.

A) Pie chart showing the percentage of ion transporters among the upregulated proteins in the L22* strain (left, n = 21). The small pie chart illustrates the percentage of ion transporters among all measured proteins (total proteome, n = 2798).

B) Table comparing the expression of two magnesium transporters (YhdP and MgtE) and one potassium transporter (KtrA) in WT, WT in the presence of spectinomycin, L34 deletion strain (ΔL34), and L22 loop duplication strain (L22*). The color bar (bottom) illustrates the fold change in protein expression.

C) Relative fold change (35 ± 9%) of intracellular magnesium content in the L22* strain compared to wild-type. For details on the ICP-OES measurements, please see the methods section.

See also Table S2.