Fig. 5 |. Convergent transcription is required for bidirectional termination in vitro.

a, SEnd-seq data track for the yoaJ-yeaQ gene pair showing an overlapping TTS. Data are representative of three independent experiments. b, Schematic of DNA templates harboring the yoaJ-yeaQ overlapping TTS region that are used for the in vitro transcription assay. c, Gel showing the RNA products transcribed from the different templates shown in (b) in the absence or presence of NusA. Data are representative of three independent experiments. d, Quantification of the fraction of readthrough transcripts for the different templates. Data are mean ± s.d. from three independent experiments. P values were determined by two-sided unpaired Student’s t-tests. e,f, SEnd-seq data track for part of the yeaQ gene (e) and DNA templates derived from this region that lacks a terminator sequence (f). The templates contain either one or two promoters to allow unidirectional or convergent transcription, respectively. g, Gel showing predominant readthrough for unidirectional transcription (Forward and Reverse templates) and heterogeneous RNA products for convergent transcription (Dual template). Data are representative of three independent experiments.