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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Oncogene. 2019 Aug 12;38(43):6926–6939. doi: 10.1038/s41388-019-0939-7

Fig. 2.

Fig. 2

Regulation of mitochondrial protein folding by LonP1. a Mitochondrial extracts of PC3 cells transfected with control non-targeting siRNA (siCtrl) or LonP1-directed siRNA (siLonP1) were prepared and replicate aliquots were solubilized in 0.05%, 0.2% or 2% Triton X-100 followed by fractionation using HPLC gel filtration. Chromatogram of the 0.05% extract. The positions of molecular weight standards are indicated. The void volume (>5 million Da) is the peak centered at 39 minutes. b The conditions are as in a, and the indicated HPLC void volume fractions were analyzed by SDS gel electrophoresis followed by silver staining. The detergent concentrations per each condition are indicated. Representative experiment. c and d PC3 cells were transfected with siCtrl or siLonP1 and detergent (NP-40)-insoluble proteins were analyzed by Western blotting (c) and quantified by densitometry (d). C, mitochondrial oxidative phosphorylation complex. Representative experiment (n=2). e Mitochondrial extracts from PC3 cells transfected with siCtrl or siLonP1 were solubilized in 1% digitonin and analyzed by native blue gel electrophoresis. The position of mitochondrial oxidative phosphorylation complexes is indicated. Marker, molecular weight markers.