Figure 1.
H&E and immunofluorescence staining of skin biopsies before and after STS procedure. Skin biopsies were collected from taped and non-taped skin area. Representative images for skin samples from healthy control subjects and AD patients are shown. Skin biopsies from normal healthy subjects, AD-lesional, and AD-nonlesional skin were stained with a standard H&E staining protocol (a). Immunofluorescence staining for FLG (red, b) and CDSN (red, c) are shown. Wheat germ agglutinin-conjugated fluorescein isothiocyanate (green) was used to stain the cytoskeleton. Arrows point to FLG or CDSN staining. All images were collected at original magnification × 400. Bar = 50 μm.