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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Aug 23;1861(11):183050. doi: 10.1016/j.bbamem.2019.183050

Figure 2. Ability of whole H. pylori lipid extracts to form vesicles with ordered state bilayers or co-existing ordered and disordered domains, as assayed by fluorescence anisotropy and FRET.

Figure 2.

(A) DPH fluorescence anisotropy. (B) FRET experiments. Lipid dispersions were composed of: (Squares) H. pylori strain JP26, (Circles) H. pylori strain JP26 cultured with supplemental cholesterol, (Diamonds) H. pylori strain JP26 plus 20 mol% cholesterol, and (Triangles) DOPC. F/Fo is the ratio of fluorescence in vesicles with donor (0.1 mol% pyrene-DPPE) and acceptor (5 mol% rhod-DOPE) to that in vesicles containing only donor, after background values subtracted. In this and the following three figures, lipids were dispersed in PBS at a total concentration of ~50 μM, and mean values from three samples and standard deviations are shown. P-values for difference of mean values at lowest and/or highest temperature calculated using Student’s t-test. ** p < 0.01, *** p < 0.001