A. Representative photomicrograph showing POMC-immunoreactivity (POMC-ir) in ARH neurons of control mice. B-D. Representative photomicrographs showing POMC innervation in different hypothalamic areas of control mice. E. Representative photomicrograph showing POMC neurons in the ARH of POMC GHR KO mice. F-H. Representative photomicrographs showing POMC innervation in different hypothalamic areas of POMC GHR KO mice. I-N. Representative photomicrographs showing AgRP innervation in different hypothalamic areas of control (I-K) and POMC GHR KO mice (L-N). Abbreviations: 3V, third ventricle; ARH, arcuate nucleus of the hypothalamus; DMH, dorsomedial nucleus of the hypothalamus; LHA, lateral hypothalamic area; PVH, paraventricular nucleus of the hypothalamus. Scale bars: A = 100 μm; B = 200 μm.