Fig. 5.

Expression levels of representative Fe-related genes in OsbHLH058 overexpression plants. Transcript levels in roots of OsbHLH058 overexpression (b058OX) lines 2 and 4 and non-transformants (NT) were quantified by RT-PCR, after 7 days of hydroponic culture under Fe sufficiency (a) or deficiency (b). Transcript abundance was normalized against the rice α-2 tubulin transcript level and expressed as a ratio relative to the levels in the +Fe NT roots. Means ± SD (n = 3) are shown. Asterisks indicate significant differences from the NT value at each condition (two-sample Student’s t-test; *P < 0.05; **P < 0.01)