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. 2019 Oct 25;9:15349. doi: 10.1038/s41598-019-51855-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of airflow limitation severity (GOLD 1–4 classification assessment) on eATP concentration. (a) eATP concentration was measured in EDTA plasma of control subjects and COPD patients subdivided by severity of airflow limitation into GOLD 2, GOLD 3 and GOLD 4 groups. (b) COPD patients were subdivided into GOLD 2–4 stages and compared to healthy individuals based on their smoking status (healthy non-smokers and healthy smokers). Data are shown as median with interquartile range for all the groups. Kruskal-Wallis test showed there was a significant difference between the groups (P < 0.001 for both a,b), and post-hoc analysis was performed. In COPD patients, a statistically significant increase in GOLD 3 in comparison to GOLD 2, and in GOLD 4 in comparison to GOLD 2 and GOLD 3 stages was also found. ^•Statistically significant difference in comparison to total controls (a) or control non-smokers (b); *statistically significant difference in comparison to control smokers (b).