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. 2019 Oct 25;9:15375. doi: 10.1038/s41598-019-51816-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in αE-catenin cysteine labeling upon shearing in the presence of F-actin. (A) Schematic representation of application of shear force to the αE-catenin/F-actin complex in a cone-plate rheometer. N-terminally His₈-tagged αE-catenin is coupled to a Ni²⁺-NTA coated glass slide in the presence of F-actin, the cysteine-labeling agent (mBBr; yellow stars) and 50% glycerol. (B) Labeling of the αE-catenin/F-actin complex under shear. Data are from 5 technical replicates and 5 independent protein preparations. p-values ≤ 0.01 are indicated with two stars (**). (C) Proximity of C438 to the M_II-M_III linker, shown in red.

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