Fig. 5.

DTR alters gut microbiota composition. a Histopathological gut inflammation score of EAE mice on d14 post-immunization (+trp: n = 10; −trp: n = 10). b–d Profiling of microbial communities by 16S rRNA gene sequencing for mice from a. b Principal coordinate analysis (PCoA; Bray-Curtis) on colonic microbial communities. c Relative abundance of significant bacterial indicator genera depicted by a heatmap. d Predicted functional categories as determined by PICRUSt. e Analysis of trp levels in plasma, feces, and ileal contents of EAE mice on d13 post-immunization (+trp: n = 5; −trp: n = 5). Statistics: Mann–WhitneyU-test for a–d; unpaired two-tailed Student’s t-test in e. In d the Mann–WhitneyU-test was performed for pair-wise comparisons with the control group and p values were corrected according to Benjamini–Hochberg. Each dot represents one individual mouse. Data are presented as mean ± SEM. Source data are provided as a Source Data file or in Supplementary Data 2–4