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. 2019 Oct 25;9:15367. doi: 10.1038/s41598-019-51873-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP in tube. (A) Gel electrophoresis analysis of products of LAMP reactions conducted in tube with varying starting copy numbers of Mtb gDNA (10⁶ copies up to 10 copies). The numbers on top of the lanes indicate the starting copy number of the template. N- No template control, M- DNA marker and D- digested LAMP amplicons. Reaction time was 40 minutes. (B) Specificity of the LAMP assay tested against non-Mtb targets. +: Positive control (Mtb gDNA), 1: Human gDNA, 2: E. coli gDNA, 3: Mycobacterium smegmatis gDNA, 4: plasmid with hepatitis C insert, 5: plasmid with dengue insert. (C) Real-time amplification curves for LAMP in tube. Reaction time was 80 minutes. (i–iv) denote lanes from different gels. Full-length gels are presented in Supplementary Fig. S9 (i), (ii), (iii) and (iv).