Fig. 2.

Wnt5a induces ROR1-dependent phosphorylation of cortactin and enhances migration of breast-cancer PDX cells. a Immunoblot analysis of lysates prepared from serum-starved PDX5 (representative of three PDXs) that subsequently were treated with Wnt5a (100 ng/ml) for the times indicated above (in minutes). b Columns indicate the mean relative tyrosine phosphorylation of cortactin at Y421 (pCortactin) (±S.D.) for PDX4, PDX5, and PDX6 treated for 0, 1, or 5 min with Wnt5a (P < 0.05, two-tailed Student’s t test). c Immunoblot analysis of lysates prepared from serum-starved PDX5 (representative of three PDXs) that subsequently were treated with Ctrl-IgG or cirmtuzumab (10 μg/ml), without (−) or with (+) Wnt5a, as indicated above. d Columns indicate the mean relative pCortactin (±S.D.) for PDX4, PDX5, and PDX6 cells treated with Ctrl-IgG or cirmtuzumab for 2 h, and subsequently treated for 5 min without (−) or with (+) Wnt5a, as indicated below (P < 0.01, Student’s t test). e Immunoblot analysis of lysates of PDX4 harvested from mice treated with Ctrl-IgG or cirmtuzumab (10 mg/kg), as indicated on top, and probed for pCortactin or Cortactin, as indicated on the left. f Columns indicate the mean relative pCortactin (±S.D.) for PDX3, PDX4, and PDX5 (P < 0.01, two-tailed Student’s t test). g Columns indicate the mean cell migration at 10 h (±S.D.) in the absence (−) or presence (+) of exogenous Wnt5a (200 ng/ml) for serum-starved PDX4, PDX5, and PDX6 that were treated with Ctrl-IgG or cirmtuzumab (10 μg/ml). Data are from three independent experiments (P < 0.05; P < 0.01, Student’s t test)