Fig. 4.
Proline at 841 of ROR1 is critical for enhancing development of metastatic foci of MCF7–ROR1. a HE staining of lung tissue from a representative tumor-bearing mouse engrafted with MCF7 or MCF7-expressing ROR1 (MCF7–ROR1) or the mutant form of ROR1, ROR1P841A (MCF7–P841A) at 1 week after intravenous (i.v.) tail-vein injections of equal numbers of viable cells. Green arrows indicate metastatic foci (objective: 40×). b Each symbol represents the number of metastatic foci that were found in the lungs of each animal in the groups indicated below (mean ± S.D., n = 5). P < 0.01; P < 0.05, two-tailed Student’s t test. c HE staining of lung tissue of mice injected as in (a), but 3 weeks after i.v. injection of equal numbers of viable cells, as indicated on top. Green arrows indicate metastatic foci (objective: 40×). d Each symbol represents the number of metastatic foci found in the lungs of each mouse per group (mean ± S.D., n = 5). P < 0.001; P < 0.01, as assessed by two-tailed Student’s t test. e HE staining of lung tissue from a representative tumor-bearing mouse injected i.v. with MCF7–ROR1 cells 1 week earlier and treated with nonspecific human IgG (IgG) or cirmtuzumab (10 mg/kg), as indicated on top. Green arrows indicate metastatic foci (objective: 40×). f Each symbol represents the number of metastatic foci that were found in the lungs of each animal in each group (mean ± S.D., n = 5) (P < 0.05, two-tailed Student’s t test). g HE staining of lung tissue from a representative tumor-bearing mouse injected i.v. with MCF7–ROR1 cells 3 weeks earlier and treated with nonspecific human IgG (IgG) or cirmtuzumab (10 mg/kg), as indicated on top. Green arrows indicate metastatic foci (objective: 40×). h Each symbol represents the number of metastatic foci that were found in the lungs of each animal in each group (mean ± S.D., n = 5 (P < 0.01, two-tailed Student’s t test)).