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. 2019 Oct 25;9:15378. doi: 10.1038/s41598-019-51591-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The 13 landmarks used for morphometric analyses in juvenile (a), adult male (b), and adult female (c) Pimephales promelas. Traditional morphometric equivalents are: standard length (1‒6), total length (1‒8), body depth (3‒10), dorsal fin base width (3‒4), caudal fin base height (5‒7), eye diameter (12‒13), head height (2‒11), and caudal peduncle length (4‒5). Size standards are displayed in the upper right of each figure.