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. 2019 Oct 25;9:15343. doi: 10.1038/s41598-019-51326-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amplified melting curves for the detection of the MTBC lineages. The graphs show the difference of the melting curves obtained by HRM analysis. Each line indicates a different sample. (A,B) Curves obtained by Multiplex PCR; (A), blue lines represent lineage 2; purple lines represent lineage 3; and red lines indicate lineage 4; (B), pink lines represent lineage 1; green lines represent lineage 6. (C,D) Curves obtained by Uniplex PCR; (C), brown lines indicate lineage 5; (D), yellow lines represent M.bovis lineage. In all cases black lines indicate a wild type genotype.