Table 1.
Specific lineage primers used in the study.
| MTBC Lineage* | SNP position† | Nucleotide change‡ | Gene | Primers 5′-3′ | Amplicon size | Reference |
|---|---|---|---|---|---|---|
| 1 | 115499 | T/G | Rv0101 | F-ATAATATTGCGTCGGTGTTGG | 81 bp | This study |
| R-TTATATATTAATGGGCAGGCC | ||||||
| 2 | 3304966§ | G/A | Rv2952 | F-TGTTACCCGCACTTTCGGCGTTT | 80 bp | This study |
| R-AGGTCGGCGTATGGGAGGTA | ||||||
| 3 | 4266647§ | A/G | Rv3804c | F-GCGACATACCCGTGACGGC | 92 bp | This study |
| R-CGTTGAGATGAGGATGAGGG | ||||||
| 4 | 2154724 | A/C | Rv1908c | F-CCGAGATTGCCAGCCTTAAG | 64 bp | 5 |
| R-GAAACTAGCTGTGAGACAGTC | ||||||
| 5 | 456731 | C/T | Rv0380c | F-GCATCGTGTCCGAAGTTCTC | 68 bp | This study |
| R-ATCATCGCCGACATCGATAC | ||||||
| 6 | 378404 | G/A | Rv0309 | F-CCGACAGCGAGAACCTGC | 54 bp | 13 |
| R-CCATCACGACCGAATGCTT | ||||||
| M.bovis | 2831482 | T/G | Rv2515c | F-GTGTTGCTGTCGATGACGC | 91 bp | This study |
| R-ACTGGTACCGCAATACCGTC |
*Nomenclature proposed by Comas et al.32.
†Genomic position on the H37Rv reference genome (NCBI, NC_000962.3).
‡Allelic change in the reference genome.
§Mutation previously described by Fenner et al.33.