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. 2019 Oct 25;9:15296. doi: 10.1038/s41598-019-51773-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TMX2 knockdown decreased importin-β-dependent nuclear transport of protein. The expression plasmid for NES-2xVenus-SV40 NLS (A) or NES-2xVenus-PTHrP NLS (B) was co-transfected with control si-RNA or si-TMX2 into HEK293 cells. After 48 h, cells were fixed and incubated with anti-TMX2 antibody, followed by secondary antibody labeling with Dylight 647. The nucleus/cytosol ratio of Venus fluorescence was measured in about 30 cells. Values are the means ± S.D. **p < 0.01 compared with the si-control.