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. 2019 Oct 25;9:15283. doi: 10.1038/s41598-019-51689-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and expression of integrin α11 variants in C2C12 cells. (A) Schematic illustration showing the amino acid sequences in the linker and the cytoplasmic tail of _Huα11 variants. (B) Western blot showing total protein expression of _Huα11-EGFP in C2C12 cells transfected with _Huα11-WT-EGFP and _Huα11-1171-EGFP (full size immunoblot is shown in supplementary) (C). FACS analysis of the cell surface expression α11 in _Huα11-WT, _Huα11-1171 and mock transfected cells (Mock).