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. 2019 Oct 25;9:15309. doi: 10.1038/s41598-019-51834-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A simplified representation of the wind tunnel assay (not to scale) is given in (A). In this example, an individual fly is exposed to an odour plume consisting of a mixture of vinegar (dark brown) and a repellent (yellow). (B) The landing rates on 1% and 5% vinegar by themselves. The landing rates on 1% vinegar (lights colours - finely dashed line) and 5% (dark colours - coarsely dashed line) in combination with (C) benzaldehyde, (D) 1-octen-3-ol, (E) geosmin and (F) phenol are given with their predicted linear regression lines based on a binomial GLM and their 95% confidence intervals. The actual landing rates on each combination of either 1% (light circles) or 5% (dark circles) vinegar with the repellents are presented with their 95% confidence intervals. The values of landing (1.00) or not landing (0.00) on the odour release point are jittered vertically and horizontally to visualize the binomial data.