Fig. 3.

PIGBOS interacts with the ER protein CLCC1. a Analysis of the PIGBOS-FLAG IP-MS to remove false positives and background proteins yielded CLCC1, an ER-resident protein, as a PIGBOS interacting protein. b Co-immunoprecipitation of PIGBOS-FLAG enriched CLCC1 from HEK293T total cell lysates. c A PIGBOS-APEX construct was used to proximity label the proteome and determine whether PIGBOS and CLCC1 are near each other in living cells. d Enrichment of CLCC1 in PIGBOS-APEX labeling proteome indicated that it was biotinylated and, therefore, in proximity to PIGBOS-APEX. e Replacement of the PIGBOS C-terminal region with 3 × GFP11 (PIGBOS-ΔC-3 × GFP11-FLAG) prevented enrichment of CLCC1