Fig. 2. BETd-260 potently induces degradation of BET proteins in OS cells.

a MNNG/HOS and b Saos-2 cell lines were treated with BETd-260, HJB-97, or JQ1 as indicated for 24 h. The protein levels of BRD2, 3, and 4 were examined by western blotting analysis. Tubulin was used as a loading control. c The MNNG/HOS cell line was treated with BETd-260 at 30 nM for different times. The protein level of BRD2, 3, and 4 were examined by western blotting analysis. Tubulin was used as a loading control. d MNNG/HOS cells were pretreated with MG-132 (2000 nmol/L), MNL4924 (500 nmol/L), or HJB-97 (3000 nmol/L) for 1 h, followed by treatment with BETd-260 (10 nM) for 2 h. The protein levels of BRD2, 3, and 4 were examined by western blotting analysis. Tubulin was used as a loading control. Data are representative of three independent experiments