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. 2019 Oct 25;10(11):813. doi: 10.1038/s41419-019-2047-6

Fig. 4. TETILA regulates MMP-9 gene expression.

Fig. 4

a Relative levels of MMP-9 mRNA assayed by RT-PCR in HaCaT cells transfected with LNA#1 or LNA#2 and treated with BSA or AGEs. b Representative microscopy images showing the expression of MMP-9 (red) with nuclear staining by DAPI (blue) in HaCaT cells transfected with LNA#2 and treated with BSA or AGEs for 72 h. c–f ChIP assay showing RNAP II (c, d) and p300 (e, f) occupancy at the MMP-9 locus after knockdown of TETILA in BSA or AGEs-treated HaCaT cells. g Luciferase reporter assays for HaCaT cells transfected with reporter plasmids containing truncated MMP-9 promoters and transfected with TETILA or vector for 24 h. Data are presented as the means ± SD of three independent experiments. **P < 0.01 vs. the corresponding control group