Fig. 6. TETILA binds to TET2 and TDG to induce promoter demethylation.

a Relative levels of MMP-9 in HaCaT cells infected with Ad-TETILA or Vector followed by treatment with the BER inhibitor (CRT) for 48 h. DMSO was used as a solvent control. b Relative levels of MMP-9 assayed by RT-qPCR in HaCaT cells infected with Ad-TETILA and co-transfected with siRNA against TDG (siTDG) or TET2 (siTET2). c, d DNA methylation analysis of the MMP-9 promoter in HaCaT cells after infected with Ad-TETILA (c) or TETILA overexpression and transfected with siTDG or siTET2 (d). e, f RIP assays showing interactions between TDG (e, P = 0.004) or DNMT1 (f) and TETILA in HaCaT cells treated with AGEs. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **or ^#P < 0.01 vs. the corresponding control group