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. 2019 Oct 25;9:15336. doi: 10.1038/s41598-019-51703-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Let-7 activity is high during mitosis in a human embryonic retinoblast line. miRNA activity assessed using miRNA sensors in HER10 cells treated with the microtubule polymerization inhibitor Nocodazole. (A,B) HER10 cells transfected with let-7 sensor. Approximately 25% of cells were GFP+ in untreated samples (A,A’), but the number of GFP+ cells decreased when cells were arrested in mitosis by treatment with Nocodazole (B,B’; p = 0.0034, n = 3). An increase in PH3+ cells confirms mitotic arrest in Nocodazole treated samples (compare A” and B”). (C,D) HER10 cells transfected with miR-1 sensor. The number of GFP + HER10 cells did not change when miR-1 sensor transfected cells were treated with Nocodazole (compare C’ and D’), despite a significant increase in PH3+ cells in Nocodazole- treated samples (compare C” to D”). (E) Quantification of the proportion of GFP+ cells in (A–D), relative to untreated control samples. n = 3. Scale bar in (A) = 50 μm.