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. 2019 Oct 25;9:15362. doi: 10.1038/s41598-019-51724-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Timelines for the two types of experiments in this study. (a) In one protocol, viral suspensions or vehicle were delivered via the PSCC at 6 wks, exposed to noise 3 wks later, allowed to recover for 2 wks, then assessed for cochlear function and/or sacrificed for tissue harvest. (b) In the second protocol, animals were exposed to noise at 9 wks, and viral suspensions were delivered via the PSCC within 4.5 hrs post-exposure. Animals recovered for 3 wks, then assessed for cochlear function and sacrificed for tissue harvest.