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. 2019 Oct 25;9:15362. doi: 10.1038/s41598-019-51724-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Virally mediated increases in cochlear NT3 expression, as assessed by qRT-PCR. (a) NT3 mRNA levels in injected and contralateral cochleas at post-injection days 1, 2.5, 5, 10, 21 and 40, normalized to mean levels in the contralateral ears. (b) To assess contralateral spread of the virus, NT3 mRNA levels were measured in both ears at 21 days post-injection and normalized to levels in uninjected controls. Histograms show means and SEMs; individual cases are shown by the colored circles in b.