Table 1.
DNA quality and yield in lysates prepared with different concentrations of SDS, different amount of tissue and different time duration
| Parameters | DNA Purity (A260/A280) | DNA Concentration (ng/μl) a | |
|---|---|---|---|
| SDS conc. (%) | 0.5 | 1.03 | 451.67 ± 8.82 |
| 1.0 | 1.01 | 768.33 ± 6.01 | |
| 1.5 | 1.03 | 376.67 ± 4.41 | |
| 2.0 | 1.04 | 252.17 ± 1.48 | |
| 2.5 | 1.03 | 438.33 ± 14.24 | |
| 3.0 | 1.04 | 261.17 ± 21.17 | |
| 3.5 | 1.05 | 218.33 ± 3.33 | |
| 4.0 | 1.06 | 260.17 ± 3.06 | |
| 4.5 | 1.05 | 326.67 ± 1.67 | |
| 5.0 | 1.04 | 446.67 ± 14.81 | |
| Amount of tissue | One disc (~ 1.6 mg) | 1.06 | 420.00 ± 10.08 |
| Two disc(~ 3.2 mg) | 1.04 | 760.00 ± 6.75 | |
| Time durations | 10 min | 1.08 | 876.67 ± 37.12 |
| 20 min | 1.05 | 808.33 ± 6.01 |
aDNA concentration is represented as Mean ± SE