Table 3.
Comparison of rHTTP method with few commercially available kits
| Kits/methods | Time required per sample | Instrumentation | Cost |
|---|---|---|---|
|
DNeasy Plant Mini Kit (Qiagen) |
~ 60 min | Grinding assembly/ Mortar-pestle, Centrifuge, vortex (6 centrifugation steps) | INR 282/− per prep |
|
Synergy™ 2.0 (OPS Diagnostics) |
43 min |
Centrifuge, vortex (5 centrifugation steps) |
INR 153/− per prep |
|
Plant DNA isolation reagent (Takara) |
30 min. | Centrifuge, vortex (3 centrifugation steps) | INR 138/− per prep |
|
Phire Plant Direct PCR Kit (Thermo Fisher Scientific) |
– | Thermal cycler (sample lysis and PCR done in a single tube). Crushing and spinning required in Dilution protocol | INR 51/− per prep |
|
QuickExtract™ Plant DNA extraction solution (Lucigen) |
8 min | Heating device with temperature controller (it requires incubation at two different temperatures) | INR 40/− per prep |
| rHTTP method | 10 min | Thermal cycler/ heating device/ any means to boil water | INR 4.5/− per prep |