Table 1.
Effects of promoter driving hygromycin selection and plasmid backbone on transformation efficiency
| BackBone | Promoter driving the selectable marker gene | Number of inoculated IE | Number of independent transgenic plants | Transformation efficiency (%) |
|---|---|---|---|---|
| pGGG | Act Hyg Intron | 150 | 14 | 9 |
| pGGG | Act Hyg Intron | 175 | 27 | 15 |
| pGGG | Act Hyg Intron | 160 | 40 | 25 |
| pGGG | Act Hyg Intron | 100 | 18 | 18 |
| pGGG | Act Hyg Intron | 25 | 5 | 20 |
| pGGG | Act Hyg Intron | 25 | 6 | 24 |
| pGGG | Act Hyg Intron | 80 | 19 | 24 |
| pGGG | Act Hyg Intron | 175 | 12 | 7 |
| pAGM | Act Hyg Intron | 100 | 15 | 15 |
| pAGM | Act Hyg Intron | 100 | 12 | 12 |
| pAGM | Act Hyg Intron | 100 | 5 | 5 |
| pAGM | Act Hyg Intron | 100 | 14 | 14 |
| pAGM | Act Hyg Intron | 100 | 24 | 24 |
| pAGM | Act Hyg Intron | 200 | 14 | 7 |
| pAGM | Act Hyg Intron | 125 | 21 | 17 |
| pAGM | Act Hyg Intron | 150 | 13 | 9 |
| pAGM | Act Hyg Intron | 150 | 16 | 11 |
| pAGM | Act Hyg Intron | 80 | 18 | 23 |
| pAGM | Act Hyg Intron | 150 | 11 | 7 |
| pAGM | Act Hyg Intron | 125 | 11 | 9 |
| pAGM | Act Hyg Intron | 150 | 15 | 10 |
| pAGM | Act Hyg Intron | 150 | 10 | 7 |
| pAGM | Act Hyg Intron | 100 | 11 | 11 |
| pBract | 35S Hyg Intron | 80 | 4 | 5 |
| pBract | 35S Hyg Intron | 150 | 10 | 7 |
| pBract | 35S Hyg Intron | 100 | 5 | 5 |
| pBract | 35S Hyg Intron | 100 | 5 | 5 |
| pBract | 35S Hyg Intron | 100 | 5 | 5 |
| pBract | 35S-Hyg-Intron | 39 | 2 | 5 |
| pBract | 35S-Hyg-Intron | 50 | 3 | 6 |