Figure 1.

MmOLF and HsOLF share key structural features. (A) Superposition of MmOLF structure (dark red, 1.55 Å resolution) and HsOLF (PDB code 4WXQ, gold, 2.15 Å resolution), view of top face, RMSD = 0.73 Å. (B) Ca²⁺, Na⁺, and corresponding coordinating residues in the central cavity of MmOLF and HsOLF are nearly identical. Waters are shown as red spheres, and hydrogen bonding interactions < 3Å are shown as dashed lines. (C) Surface electrostatics demonstrate similar potentials across the top face, but increased positive charge on the bottom face of MmOLF relative to HsOLF. Surface potentials are colored negative (red, −5 kT/e-) to positive (blue, +5 kT/e-).