Table 1.
Quantification of developmental delay phenotype in wild-type background and spg-7(ad2249) mutant background
| Wild-type C. elegans animals | spg-7(ad2249) mutant | |||
| % Adults | % Adults | |||
| E. coli genotype | Day 3 | Day 4 | Day 4 | Day 5 |
| BW25113 (wild type) | 100 ± 0 (244) | NA | 100 ± 0 (195) | ND |
| fes | 100 ± 0 (244) | NA | 0 ± 0 (169) | 0 ± 0 (224), L1-arrest |
| fepG | 100 ± 0 (265) | NA | 0 ± 0 (185) | 0 ± 0 (246), L1-arrest |
| fepD | 100 ± 0 (259) | NA | 0 ± 0 (183) | 0 ± 0 (255), L1-arrest |
| fepC | 100 ± 0 (214) | NA | 0 ± 0 (186) | 0 ± 0 (247), L1-arrest |
| cyoD | 9.8 ± 3.7 (221)*,† | 83.2 ± 9 (236) | 0 ± 0 (181) | 64.6 ± 6.4 (244) |
| yafC | 100 ± 0 (240) | NA | 0 ± 0 (167) | 60.6 ± 8 (256) |
| cyoB | 0 ± 0 (233)† | 59 ± 6.3 (233) | 0 ± 0 (188) | 66.6 ± 7.2 (247) |
| cyoA | 0 ± 0 (256)† | 69.4 ± 17.2 (227) | 0 ± 0 (155) | 77.1 ± 5 (235) |
| tap | 83 ± 6.4 (251)* | 98.2 ± 0.8 (238) | 0 ± 0 (166) | 78.3 ± 20 (226) |
| cpxR | 0 ± 0 (249)† | 69.2 ± 15.4 (233) | 0 ± 0 (185) | 80.7 ± 13.4 (235) |
| stfP | 0 ± 0 (255)† | 64 ± 8.4 (248) | 0 ± 0 (149) | 74.6 ± 10.9 (250) |
| mdoC | 0 ± 0 (247)† | 72.2 ± 11.7 (229) | 0 ± 0 (156) | 75.1 ± 9.1 (215) |
| ycfk | 0 ± 0 (233)† | 72.9 ± 17.4 (221) | 0 ± 0 (181) | 78.7 ± 7.7 (208) |
| yejL | 100 ± 0 (260) | NA | 0 ± 0 (209) | 76.4 ± 12.3 (260) |
| yejK | 100 ± 0 (210) | NA | 0 ± 0 (182) | 80.3 ± 6.4 (228) |
| allA | 0 ± 0 (233)† | 52.8 ± 14.1 (241) | 0 ± 0 (172) | 81.8 ± 4.6 (236) |
| ybjX | 0 ± 0 (197)† | 56.9 ± 15.4 (230) | 0 ± 0 (178) | 71.5 ± 14.8 (225) |
| yfaE | 0 ± 0 (250)† | 55.1 ± 13.2 (240) | 0 ± 0 (184) | 82.6 ± 3.8 (236) |
| ybaW | 0 ± 0 (246)† | 63.1 ± 9.5 (255) | 0 ± 0 (192) | 80.1 ± 8 (237) |
| ycfX | 0 ± 0 (240)† | 62.9 ± 8.9 (257) | 0 ± 0 (192) | 82.4 ± 10.3 (234) |
| ygbM | 0 ± 0 (187)† | 61.4 ± 12.8 (230) | 0 ± 0 (200) | 69 ± 5.5 (224) |
| ydaN | 0 ± 0 (260)† | 63.1 ± 7.9 (232) | 0 ± 0 (194) | 79.1 ± 1.6 (220) |
| ybiB | 0 ± 0 (243)† | 57.1 ± 15.5 (236) | 0 ± 0 (199) | 76.7 ± 5.9 (227) |
| mntR | 100 ± 0 (264) | NA | 0 ± 0 (135) | 76.9 ± 9.8 (219) |
| yaaJ | 100 ± 0 (228) | NA | 0 ± 0 (159) | 66.2 ± 16.4 (231) |
| yfaY | 0 ± 0 (253)† | 61.7 ± 15.6 (245) | 0 ± 0 (188) | 73 ± 18 (247) |
| yejB | 0 ± 0 (228)† | 60.2 ± 3.7 (251) | 0 ± 0 (172) | 66.4 ± 12.2 (233) |
| yfiB | 0 ± 0 (234)† | 59.8 ± 3.6 (250) | 0 ± 0 (190) | 75.1 ± 8.1 (233) |
| tar | 100 ± 0 (242) | NA | 0 ± 0 (179) | 73.9 ± 4 (226) |
| yheT | 0 ± 0 (252)† | 64.6 ± 4.7 (234) | 0 ± 0 (154) | 77.7 ± 7.5 (233) |
| yaaU | 100 ± 0 (257) | NA | 0 ± 0 (196) | 80.4 ± 7.1 (251) |
| aroH | 100 ± 0 (231) | NA | 0 ± 0 (172) | 76.8 ± 4 (235) |
| aes | 0 ± 0 (232)† | 63.9 ± 5.3 (239) | 0 ± 0 (181) | 76.3 ± 2.1 (234) |
| cyaA | 100 ± 0 (257) | NA | 0 ± 0 (165) | 77.6 ± 2 (223) |
| hlyE | 100 ± 0 (227) | NA | 0 ± 0 (159) | 75.1 ± 3.4 (237) |
| yceF | 0 ± 0 (238)† | 72.47 ± 3.1 (236) | 0 ± 0 (193) | 78.2 ± 2 (252) |
| wbbI | 0 ± 0 (236)† | 63.5 ± 8.8 (239) | 0 ± 0 (183) | 69.7 ± 12 (253) |
| ssuE | 100 ± 0 (244) | NA | 0 ± 0 (195) | 77 ± 6.5 (232) |
| cspH | 100 ± 0 (270) | NA | 0 ± 0 (163) | 71 ± 6.6 (230) |
| yaiF | 100 ± 0 (251) | NA | 0 ± 0 (198) | 75.5 ± 6.4 (236) |
| rhsD | 100 ± 0 (255) | NA | 0 ± 0 (169) | 82.6 ± 0.65 (236) |
| rhsB | 100 ± 0 (265) | NA | 0 ± 0 (182) | 80.4 ± 5.2 (244) |
| ydcC | 0 ± 0 (240)† | 67.7 ± 15.7 (239) | 0 ± 0 (183) | 67.7 ± 13.2 (246) |
| cspD | 100 ± 0 (215) | NA | 0 ± 0 (137) | 77.8 ± 7.4 (229) |
| eutD | 100 ± 0 (256) | NA | 0 ± 0 (165) | 78.4 ± 4.6 (242) |
Synchronized L1 wild-type animals were inoculated onto NGM media plates seeded with individual Keio E. coli mutant strains and incubated at 20 °C. The number of adult animals and the total number of animals were counted on day 3 and day 4 of feeding wild-type animals on Keio E. coli mutant strains. Data from 3 independent trials were collected; the average of percentage of adults and the SD are shown. The total number of animals counted in all 3 independent trials is shown in parentheses. Boldface indicates L1-arrest phenotype was observed.
Compared to wild-type animals fed on BW25113 on day 3, unpaired t test, P < 0.0001.
Since there were no adults or only few adults on these plates, the plates were scored on day 4 for the presence of adults. While there were no adults on day3, many of the wells had animals that reached adulthood on day 4. NA, not applicable since all of the animals were adults on day 3 itself. For columns 4 and 5, synchronized L1 spg-7(ad2249) mutant were inoculated onto NGM media plates seeded with individual Keio E. coli mutant strains and incubated at 20 °C. The number of adult animals and the total number of animals were counted on days 4 and 5 of feeding spg-7(ad2249 animals on Keio E. coli mutant strains. Data from 3 independent trials were collected; the average of percentage of adults and the SD are shown. The total number of animals counted in all 3 independent trials is shown in parentheses.