Fig. 2.

Effect of temperature and Adh genotype on ethanol tolerance in transgenic flies. Adh-null strains were transformed with a naturally occurring Slow allele (blue), the Slow allele modified with mutation K192T (red), or a naturally occurring Fast allele containing T192 and linked polymorphisms (green). Larvae (A and B) and adult females (C and D) were assayed for survival in the presence of increasing ethanol concentrations at different temperatures. Circles and error bars show the mean and SEM of the fraction of individuals dead in each treatment. For larvae, survival until eclosion was measured in 8 to 10 replicates of 30 larvae each per treatment; for adults, survival after 48 h of exposure was measured in 4 to 8 replicates of 25 to 30 individuals each per treatment. For each genotype, the LD₅₀ is shown, with SE. Asterisks, significant differences between genotypes (P < 0.05, Wald’s test).