Fig. 5.

Multiplexed sADPL activity profiling in patient-derived tumor samples. (A) Schematic depiction of sADPL profiling of cancer spheroids and primary tumor and metastatic tumor tissues. (B) Heatmap depiction of a representative, multiplexed sADPL profiling experiment detecting 6 active enzymes from 2 tumor compartments in 18 patients. All data points are z-scores normalized from 3 technical replicates from 1 biological replicate. Individual activity measurements below the detection threshold are shown in black. (C) Metastatic/primary tumor activity ratio co-occurrence for each enzyme target measured across the 18-patient cohort. Active enzymes that show higher co-occurrence are yellow on the relative scale. (D) Within-patient activity measurements between primary and metastatic tumor sites reveals that CTSB and CTSL activities are significantly altered across the patient cohort. *P < 0.05, Wilcoxon signed-rank t test. (E) Correlation between patient survival and metastatic/primary tumor activity ratios demonstrates the presence of significant negative correlations for the enzymes shown. Each point represents an individual patient and corresponding mean activity ratio from 3 technical and 2 biological replicates.