Fig. 2.

IL-10–producing B cells in PATs. Adult WT B6 mice were used. (A) Flow cytometry gating schemes for subset distribution among IL-10–producing B cells in PATs are shown. (B and C) SVFs from PATs of mice with the indicated genotypes were cultured in the presence of the protein transport inhibitor monensin under the indicated stimulations. Cells were then examined for IL-10⁺ B cells (Left) and subset distribution among IL-10⁺ B cells (Right). Summary of 3 independent experiments is shown in B (n = 8 to 12), and summary of 2 independent experiments is shown in C (n = 6). (D) WT B6 mice were either left untreated (Left) or intraperitoneally injected with LPS (Right), and were killed 3 d later. SVFs from pooled PATs were sorted by flow cytometry to obtain subsets of B cells. Total RNAs from the sorted cells were used to detect IL-10 transcripts by real-time PCR. Results from 2 independent experiments are shown (AU, arbitrary unit). (E) SVFs from pooled PATs of WT B6 mice were sorted by flow cytometry to obtain subsets of B cells. Cells were cultured in the absence or presence of LPS for 48 h. IL-10 in the culture supernatant was then measured. ^#P < 0.05 and *P < 0.01 for all of the panels.