Fig. 4.

Postnatal maintenance of the B cell compartment in PATs. Adult WT B6 mice were used. (A) Flow cytometry gating schemes for B cell subsets of donor/recipient origins are shown. (B) B cells from PerC of CD45.1⁺ mice were injected into the indicated body locations of CD45.2⁺ mice. Donor cells among subsets of B cells were examined in the indicated adipose depots (summary of 2 to 3 independent experiments, n = 4 to 8). (C) CD45.1⁺ and CD45.2⁺ mice were joined by parabiosis. CD45.1⁺ cells among subsets of B cells in the indicated body locations of CD45.2⁺ mice are shown (summary of 3 independent experiments, n = 12 pairs). *P < 0.01 for all panels.