Impact of cytokines on the B cell compartment in PATs. Adult mice were used. (A) Transcripts of the indicated genes in the indicated adipose depots of WT B6 mice were examined (summary of 2 independent experiments, n = 8; AU, arbitrary unit). (B and E) Frequencies of B cells (Left) and their subsets (Right) in PATs of mice with the indicated genotypes are shown (summary of 2 independent experiments, n = 9 to 12). (C) Frequencies of CD5+ B cells in the indicated adipose depots of mice with indicated genotypes are shown (summary of 2 independent experiments, n = 12). (D and F) SVFs from PATs of mice with the indicated genotypes were treated as described in the legend for Fig. 2B, and examined for IL-10+ B cells (Left) and subset distribution among IL-10+ B cells (Right). Summaries of 2 independent experiments (n = 9 to 12) are shown. #P < 0.05 and *P < 0.01 for all of the panels.