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. 2019 Oct 7;116(43):21529–21538. doi: 10.1073/pnas.1914999116

Fig. 1.

Fig. 1.

Characterization of DNAJB6b and the ΔST-DNAJB6b deletion mutant. (A) Domain architecture of the canonical isoform DNAJB1 and DNAJB6b, highlighting the J domain (JD), the flexible G/F linker, the C-terminal domains (CTDx), and the dimerization domain (D). DNAJB6b lacks the CTD-II and dimerization domains and has a low-complexity S/T region immediately N-terminal to the CTD domain. (B) Dynamic light scattering profile of 60 μΜ DNAJB6b. (C) Analytical size exclusion chromatography profiles of 100 μΜ DNAJB6b (blue) and ΔST-DNAJB6b (lilac). (D) Overlay of the TROSY 1H-15N HSQC spectra of 2H/15N-labeled DNAJB6b (blue) and ΔST-DNAJB6b (lilac) recorded at 600 MHz and 25 °C.