Fig. 4.

Conformation of β-catenin by itself and as part of the ^hB^dE or ^dA^hB^dE complex. (A) SEC-SAXS data of β-catenin (black squares; reveal the conformation of β-catenin alone in solution) and contrast-matching SANS data of the ^hB^dE complex in 100% D₂O buffer (red squares; reveal the conformation of ^hβ-catenin in ^hB^dE) and of the ^dA^hB^dE complex in 100% D₂O buffer (blue squares; reveal the conformation of ^hβ-catenin in the ^dA^hB^dE complex). The lines are fits to the experimental data for generating the P(r) functions. SEC-SAXS profile of β-catenin in solution is shown in SI Appendix, Fig. S6. (B) P(r) of β-catenin in solution (black) and as part of the ^hB^dE complex (red) or the ^dA^hB^dE complex (blue). (C) Dimensionless Kratky plots of the SAXS and SANS data shown in A. (D–F) The 3D molecular shape of β-catenin in solution from SEC-SAXS (D) and β-catenin in the ^hB^dE complex from SANS in 100% D₂O (E) and in the ^dA^hB^dE complex (F) from SANS in 100% D₂O buffer. The 3D shapes were generated using the program Gasbor (68).