Fig. 7.

Reduced MYBS2 expression up-regulates αAmy3 that enhances abiotic stress tolerance in rice. (A and B) Total RNAs were extracted from seedlings for qRT-PCR analysis using αAmy3-, αAmy7- and MYBS2-specific primers. The value of mRNA level in the nontreated control (CK) was set to 1×, and all other values were calculated relative to this value. (A) Ten-day-old seedlings of sWT rice were treated with the indicated abiotic stress. (B) Seeds of sWT, MYBS2 (full-length or truncated) Ox and Ri lines were germinated in medium with or without 400 mM sorbitol (S) for 8 d. n = 60. (C and D) Shoot length of seedlings in experiment (B) was determined and plant morphology was photographed on day 8. (E–I) Transgenic rice overexpressing Ubi:αAmy3 or αAmy3:αAmy3 were used in the experiment. Seeds were germinated in medium without sorbitol (CK) or with 400 mM sorbitol (S). (E) Total RNA were extracted from 8-d-old seedlings for qRT-PCR analysis using aAmy3-specific primers. (F and H) Germination rates were determined daily up to day 5. (G and I) Shoot length was determined and plant morphology was photographed at day 8. n = 30. Error bars represent SD. Asterisks indicate significant differences (Student’s t test, **P < 0.01, ***P < 0.001).