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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biochimie. 2019 Jun 15;166:161–172. doi: 10.1016/j.biochi.2019.06.010

Figure 5. Mutations of Arg 700, 701 and 708 residues to Lys do not affect processing of ProRgpB6His at the CTD in contrast to S664R substitution, which facilitates removal of the entire CTD.

Figure 5.

(A) ProRgpB6His with mutation(s) in the CTD domain were incubated with mature RgpB (molar ratio 1:100) at room temperature in gingipain activity buffer supplemented with 10 mM DTT. At various times points, aliquots were removed to follow the maturation of the enzyme by SDS-PAGE. (B) The final products obtained after 96 hours of incubation were analysed by Western blotting with pAb anti-RgpB, mAb anti-6His and pAb anti-RgpBCTD domain.