(A-F) Immunofluorescence confocal microscopy of sagittal eye sections from control (Cre-Chmp4bfx/+) mice (A, C, E) and Chmp4b-CKD mice (B, D, F) at E16.5 (A, B), P1 (C, D), and P24 (E, F) labeled with anti-CD68. CD68-positive cells were present in the embryonic and postnatal Chmp4b-CKD lens (B, D, F) and surrounding embryonic vasculature or TVL (B) but were absent from wild-type lens (A, C, E) and confined to the embryonic TVL (A). Cell nuclei were stained blue with DAPI. Scale bar: 100 μm (A-F), 50 μm (F inset).