Table 2.
Predicted regulatory effects of miRNAs whose expression is restored to youthful levels in aortas of aged mice by NMN supplementation. Shown are GO terms enriched among miRNAs differentially expressed with age in the aorta whose expression is significantly affected by NMN treatment. N = genes in each GO category, targeted by miRNAs that are differentially regulated in the aged mouse aorta. Significance was determined by Fisher’s exact test; odds ratio: (observed to expected ratio); SLPV: signed log10 P value
| GO term ID | Name of biological process/molecular function | N | Odds Ratio | SLPV |
|---|---|---|---|---|
| 6886 | Intracellular protein transport | 20 | 3.17 | 3.26 |
| 7218 | Neuropeptide signaling pathway | 7 | 7.32 | 2.54 |
| 5198 | Structural molecule activity | 6 | 9.40 | 2.45 |
| 51082 | Unfolded protein binding | 7 | 5.49 | 2.20 |
| 45778 | Positive regulation of ossification | 6 | 6.27 | 2.07 |
| 50839 | Cell adhesion molecule binding | 10 | 3.49 | 1.92 |
| 15137 | Citrate transmembrane transporter activity | 3 | inf | 1.84 |
| 48227 | Plasma membrane to endosome transport | 3 | inf | 1.84 |
| 8188 | Neuropeptide receptor activity | 3 | inf | 1.84 |
| 7217 | Tachykinin receptor signaling pathway | 3 | inf | 1.84 |
| 42594 | Response to starvation | 3 | inf | 1.84 |
| 70536 | Protein K63-linked deubiquitination | 6 | 4.70 | 1.77 |
| 71108 | Protein K48-linked deubiquitination | 6 | 4.70 | 1.77 |
| 5102 | Receptor binding | 27 | 1.82 | 1.72 |
| 90630 | Activation of GTPase activity | 10 | 2.85 | 1.71 |
| 31338 | Regulation of vesicle fusion | 7 | 3.66 | 1.68 |
| 1664 | G-protein coupled receptor binding | 6 | 3.76 | 1.52 |
| 6631 | Fatty acid metabolic process | 6 | 3.76 | 1.52 |
| 45777 | Positive regulation of blood pressure | 4 | 6.25 | 1.47 |
| 32924 | Activin receptor signaling pathway | 4 | 6.25 | 1.47 |
| 70530 | K63-linked polyubiquitin binding | 4 | 6.25 | 1.47 |
| 10863 | Positive regulation of phospholipase C activity | 4 | 6.25 | 1.47 |
| 16579 | Protein deubiquitination | 8 | 3.13 | 1.47 |
| 18107 | Peptidyl-threonine phosphorylation | 9 | 2.57 | 1.42 |
| 48015 | Phosphatidylinositol-mediated signaling | 5 | 3.91 | 1.36 |
| 7200 | Phospholipase C-activating G-protein coupled receptor signaling pathway | 5 | 3.91 | 1.36 |
| 71837 | HMG box domain binding | 5 | 3.91 | 1.36 |
| 61578 | Lys63-specific deubiquitinase activity | 3 | 9.37 | 1.33 |
| 33674 | Positive regulation of kinase activity | 3 | 9.37 | 1.33 |
| 43122 | Regulation of I-kappaB kinase/NF-kappaB signaling | 3 | 9.37 | 1.33 |
| 50995 | Negative regulation of lipid catabolic process | 3 | 9.37 | 1.33 |