Table 1.
Primer pairs (forward and reverse) used in the experiment
| Gene | Primers | Length | Code |
|---|---|---|---|
| β-2-Microglobulin (β2m) |
F: AAGTATACTCACGCCACCCA R: CAGGCGTATGTATCAGTCTC |
217 | NM_009735.3 |
| Beta actin (Actb) |
F: GAGACCTTCAACACCCCAGC R: ATGTCACGCACGATTTCCC |
263 | NM_007393.5 |
| Protein kinase B (Akt1) |
F: CCGGTTCTTTGCCAACATCG R: ACACACTCCATGCTGTCATCTT |
168 | NM_001331107.1 |
| Phosphoinositide-3-kinase (Pi3k) |
F: TAGCTGCATTGGAGCTCCTT R: TACGAACTGTGGGAGCAGAT |
119 | XM_006507441.3 |
| Mechanistic target of rapamycin kinase (Mtor) |
F: CGGCAACTTGACCATCCTCT R: TGCTGGAAGGCGTCAATCTT |
101 | XM_006539077.2 |
| Forkhead box protein O3 (Foxo3a) |
F: CGGCTCACTTTGTCCCAGAT R: GCCGGATGGAGTTCTTCCA |
106 | XM_006512806.1 |
| Growth differentiation factor 9 (Gdf9) |
F: AATACCGTCCGGCTCTTCAG R: GGTTAAACAGCAGGTCCACCAT |
97 | XM_006532220.3 |
| Anti-Mullerian hormone (Amh) |
F: TCCTACATCTGGCTGAAGTGATATG R: CAGGTGGAGGCTCTTGGAACT |
66 | XM_006513119.3 |
| Bone morphogenetic protein 15 (Bmp-15) |
F: GAGCGAAAATGGTGAGGCTG R: GGCGAAGAACACTCCGTCC |
342 | NM_009757.5 |
| Proto-oncogene receptor tyrosine kinase (Kit) |
F: CTCCCCCAACAGTGTATTCAC R: TAGCCCGAAATCGCAAATCTT |
90 | NC_000071.6 |
| Kit Ligand (Kitl) |
F: TTCGCACAGTGGCTGGTAAC R: TTCACAGCGAAGCACTCTGC |
163 | NM_013598.3 |
| Phosphatase and tensin homolog (Pten) |
F: TGGATTCGACTTAGACTTGACCT R: GCGGTGTCATAATGTCTCTCAG |
180 | NM_008960.2 |