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Description of immunostaining techniques. a Indirect immunostaining: protein-specific antibody (Ig1) recognizes the protein of interest (POI) expressed in cells. A second antibody (Ig2) directed against the first antibody (anti-mouse/rabbit/human, etc.) is labeled with a fluorescent dye. b Direct immunostaining: protein-specific antibody (Ig) recognizes a protein of interest (POI) and is labeled with fluorescent dye
