Table 1.

Summary of staining methods for SR. Different methods suitable for live-cell imaging (upper) and for fixed cell imaging (lower)

Fixed	Immunostaining	Immunostaining	Chemical tag
	Indirect (protein-specific antibody + secondary antibody) Direct (protein-specific antibody)	Fluorescent/photoactivatable proteins antibody	HaloEnzyme-Halo-Tag-fluorophore
LIVE	Fusion protein	Fusion protein (smaller peptide)	Expression System
	Fluorescent proteins (FPs) Photoactivatable fluorescent proteins (PA-FPs)	Chemical tags	Transient or stable transfection CRISP/Cas9