Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotype | Reference |
|---|---|---|
| Strain | ||
| Halomonas hydrothermalis Y2 | Halotolerant alkaliphilic strain | [26] |
| E. coli DH5α | Competent cell for cloning | |
| E. coli S17-1 | Competent cell for gene knockout | |
| Y2/ΔectD | Strain Y2 with ectD deficient | This study |
| Y2/ΔectD/ΔdoeA | Strain Y2 with ectD and doeA deficient | This study |
| Y2/ΔectD/ΔdoeA/ΔnhaD2 | Strain Y2 with ectD, doeA, and nhaD2 deficient | This study |
| Y2/ΔectD/ΔdoeA/Δmrp | Strain Y2 with ectD, doeA, and mrp deficient | This study |
| Y2/ΔectD/ΔdoeA/ΔnhaD2/Δmrp | Strain Y2 with ectD, doeA, nhaD2, and mrp deficient | This study |
| Plasmid | ||
| pEASYBlunt | Cloning vector | TransGen Biotech |
| pK18mobSacB | Kmr mobsacB | |
| pK18mobSacB/ΔectD | pK18mobSacB with ΔectD | This study |
| pK18mobSacB/ΔectD/ΔdoeA | pK18mobSacB with ΔectD and ΔdoeA | This study |
| pK18mobSacB/ΔectD/ΔdoeA/ΔnhaD2 | pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2 | This study |
| pK18mobSacB/ΔectD/ΔdoeA/Δmrp | pK18mobSacB with ΔectD, ΔdoeA, Δmrp | This study |
| pK18mobSacB/ΔectD/ΔdoeA/ΔnhaD2/Δmrp | pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2, Δmrp, | This study |