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Primers for gene knockout used in this study
| Name | Sequence |
|---|---|
| ectD-F1 | CGCGGATCCATGACAGTCGGCAATACACCTCTC |
| ectD-R1 | AAGGCAGGGCACCCGCTCGCGGTAAGCAT |
| ectD-F2 | TTACCGCGAGCGGGTGCCCTGCCTTGGTGAAA |
| ectD-R2 | CCCAAGCTTTTAAACGCCCGGCTGCCAAG |
| doeA-F1 | CGCGGATCCGTGGCCCAGTGGCGCGTTTCA |
| doeA-R1 | TTGAACACCCCGCCGGTGTGCCAGCCAC |
| doeA-F2 | TGGCACACCGGCGGGGTGTTCAAGCGCTA |
| doeA-R2 | CCCAAGCTTCTACTTCACGAACAGCTG |
The restriction sites in primers are underlined
