Fig. 5.

T007 effected on osteoblastogenesis and osteoblast-specific gene expression. a IC50 values obtained for the activity of T007 against MC3T3-E1 cells. b Effect of T007 on MC3T3-E1 on cell viability by CCK8 assays at 48 and 96 h. c ALP expression in osteoblasts and treated with various concentrations of T007 for 7 days. Mineralized extracellular matrix was detected using Alizarin Red S staining for 21 days. d The number of ALP-positive cells and the OD values obtained for mineralized matrix solutions following the treatment with T007. e Expression of the osteoblast-specific genes Runx2, ALP, OCN, COL1a, and OSX in MC3T3-E1 cells treated with various concentrations of T007 for 7 days. f, g The expression levels of RANKL, OPG, and RANKL/OPG in MC3T3-E1 cells treated with various concentrations of T007 for 0, 1, 3 or 5 days. The results were normalized to the expression levels of GAPDH. All experiments were performed at least three times. *P < 0.05 and **P < 0.01 compared with the control group