Fig. 6.

T007 promoted the expression of the osteoblast-specific genes Runx2 by inhibiting PPARγ. a-d MC3T3-E1 cells were treated with various concentrations of T007, and the expression of the indicated proteins was detected by western blotting. e-l MC3T3-E1 cells were treated with osteoblastogenic medium, and treated with 0.48 μM of T007 for the indicated day, and the expression levels of PPARγ, Runx2,β-catenin, RANKL, and OPG were evaluated by western blotting. m MC3T3-E1 cells were treated with or without 0.48 μM of T007. The expression levels of PPARγ and Runx2 were detected using immunofluorescence. n MC3T3-E1 cells were treated with osteoblastogenic medium, and treated with T007 (0.48 μM) or MG 132 (1 μM) for 7 days, and the expression of PPARγ was detected by western blotting. All experiments were performed at least three times. *P < 0.05 and **P < 0.01 compared with the control group